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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- 44-632G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-PYK2 (Tyr579) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis.
Analyzing FAK and Pyk2 in early integrin signaling events.
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
McGinnis LK, Pelech S, Kinsey WH
Molecular reproduction and development 2014 Oct;81(10):928-45
Molecular reproduction and development 2014 Oct;81(10):928-45
Analyzing FAK and Pyk2 in early integrin signaling events.
Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Wang Z, Brecher P
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Wang Z, Brecher P
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Peptide Competition. Extracts of primary chicken embryo fibroblasts expressing Pyk2 and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for two hours at room temperature and either left untreated (1-4) or treated with lambda phosphatase (5), then incubated with the Pyk2 (pY579) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab’)2 anti-rabbit IgG alkaline phosphatase (Product # ALI4405) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to Pyk2 (pY579) completely blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.
