H00002185-D01P

antibody from Abnova Corporation

Targeting: PTK2B CADTK, CAKB, FAK2, PTK, PYK2, RAFTK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002185-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002185-D01P, RRID:AB_1579340

Product name

PTK2B purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PTK2B protein.

Antigen sequence

MSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEK
EDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIREI
ITSILLSGRIGPNIRLAECYGLRLKHMKSDEIHWL
HPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDF
MESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMA
LQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGL
DLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEE
CVMKFFNTLAGFANIDQETYRCELIQGWNITVDLV
IGPKGIRQLTSQDAKPTCLAEFKQIRSIRCLPLEE
GQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDG
YCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLE
ARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGI
AREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVA
VKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLI
GIIEEEPTWIIMELYPYGELGHYLERNKNSLKVLT
LVLYSLQICKAMAYLESINCVHRDIAVRNILVASP
ECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPE
SINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLE
NKDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYD
PSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYR
TPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKL
QFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHR
HNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMR
QILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSP
LTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDR
TDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVV
KNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKL
LNKDLAELINKMRLAQQNAVTSLSEECKRQMLTAS
HTLAMDAKNLLDAVDQAKVLANLAHPPAE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PTK2B MaxPab rabbit polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PTK2B expression in transfected 293T cell line (H00002185-T01) by PTK2B MaxPab polyclonal antibody.Lane 1: PTK2B transfected lysate(115.90 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to PTK2B on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2B and ERBB2. HeLa cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-ERBB2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2B and YES1. Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)