Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC2890F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal STAT2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human STAT2 when phosphorylated at Y689 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4457-69
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4457-69
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line untreated (light orange filled histogram) or treated with Recombinant Human IFN-alpha A (Catalog # 11100-1) (dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2890F) or isotype control antibody (Catalog # IC105F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.