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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC8627G-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-STAT2 (Y689) Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human phospho-STAT2 (Y689) in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 1021D
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-alpha A (Catalog # 11100-1) for 20 minutes was stained with Rabbit Anti-Human STAT2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8627G, filled histogram) or isotype control antibody (Catalog # IC105G, not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
