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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 693302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#693302, RRID:AB_2632834
- Product name
- Purified anti-STAT2
- Antibody type
- Monoclonal
- Description
- STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT1, p48, and CREB binding protein.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W15088A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15 µg protein) from Jurkat, 293T and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-STAT2 (clone W15088A) antibody. Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
- Conjugate
- Unconjugated
