LS-B652

antibody from LSBio

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B652

Provider

LSBio

Product name

IHC-plus™ RELA / NFKB p65 Antibody (phospho-Ser529) LS-B652

Antibody type

Polyclonal

Description

Delipidated and defibrinated

Reactivity

Human

Host

Rabbit

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Top row is anti-pan p65. Bottom row is anti-pS529 showing phospho p65 staining in carcinoma cells. Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-pS529 shows phospho p65 staining in carcinoma cells.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 (code# LS-B653) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the Western blot of a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.