Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-27416 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RelA/NFkB p65 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas.
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas.
Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases.
Lee TL, Yeh J, Friedman J, Yan B, Yang X, Yeh NT, Van Waes C, Chen Z
International journal of cancer 2008 May 1;122(9):1987-98
International journal of cancer 2008 May 1;122(9):1987-98
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Santhi WS, Sebastian P, Varghese BT, Prakash O, Pillai MR
Experimental and molecular pathology 2006 Oct;81(2):123-30
Experimental and molecular pathology 2006 Oct;81(2):123-30
Cell-cycle regulators, bcl-2 and NF-kappaB in Epstein-Barr virus-positive gastric carcinomas.
Chang MS, Lee HS, Jung EJ, Kim CW, Lee BL, Kim WH
International journal of oncology 2005 Nov;27(5):1265-72
International journal of oncology 2005 Nov;27(5):1265-72
Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases.
Wittmann M, Purwar R, Hartmann C, Gutzmer R, Werfel T
The Journal of investigative dermatology 2005 Jun;124(6):1225-33
The Journal of investigative dermatology 2005 Jun;124(6):1225-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - analysis of p65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.