Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC5078P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RelA/NFkB p65 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse RelA/NF kappa B p65 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.
TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.
Galle-Treger L, Sankaranarayanan I, Hurrell BP, Howard E, Lo R, Maazi H, Lewis G, Banie H, Epstein AL, Hu P, Rehan VK, Gilliland FD, Allayee H, Soroosh P, Sharpe AH, Akbari O
Nature communications 2019 Feb 12;10(1):713
Nature communications 2019 Feb 12;10(1):713
TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.
Hurrell BP, Galle-Treger L, Jahani PS, Howard E, Helou DG, Banie H, Soroosh P, Akbari O
Cell reports 2019 Dec 24;29(13):4509-4524.e5
Cell reports 2019 Dec 24;29(13):4509-4524.e5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.