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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 44-712G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-NFkB p65 (Ser536) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of lysates prepared from HeLa cells using rabbit anti- NFKB (pS536) antibody (Product # 44-712G). HeLa cells were left untreated (lane 1) or treated with TNF-alpha and ALLN (lanes 2-5) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with the primary antibody, the membrane was incubated with no peptide (lane 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or the phosphopeptide immunogen (lane 5).
