Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-37718 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NFkB p65 (Ser276) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast and human lung carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Non-epigenetic induction of HEXIM1 by DNMT1 inhibitors and functional relevance.
Role of Nrf2 Signaling in the Regulation of Vascular BK Channel β1 Subunit Expression and BK Channel Function in High-Fat Diet-Induced Diabetic Mice.
Sharma V, Montano MM
Scientific reports 2020 Dec 3;10(1):21015
Scientific reports 2020 Dec 3;10(1):21015
Role of Nrf2 Signaling in the Regulation of Vascular BK Channel β1 Subunit Expression and BK Channel Function in High-Fat Diet-Induced Diabetic Mice.
Lu T, Sun X, Li Y, Chai Q, Wang XL, Lee HC
Diabetes 2017 Oct;66(10):2681-2690
Diabetes 2017 Oct;66(10):2681-2690
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Hela cells using NF-kappa-B p65 (pSer276) polyclonal antibody (Product # PA5-37718) (left) or the same antibody preincubated with a non-phospho peptide blocking peptide (Lane 2) and a phospho blocking peptide (Lane 3).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-NFkB p65 (Ser276) in methanol-fixed Hela cells, using Phospho-NFkB p65 (Ser276) Polyclonal Antibody (Product # PA5-37718).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-NFkB p65 (Ser276) in methanol-fixed MEF cells, using Phospho-NFkB p65 (Ser276) Polyclonal Antibody (Product # PA5-37718).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-NFkB p65 (Ser276) in paraffin-embedded Human breast carcinoma tissue using Phospho-NFkB p65 (Ser276) Polyclonal Antibody (Product # PA5-37718) (left) or the same antibody preincubated with blocking peptide (right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-NFkB p65 (Ser276) in paraffin-embedded Human breast carcinoma tissue using Phospho-NFkB p65 (Ser276) Polyclonal Antibody (Product # PA5-37718).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-NFkB p65 (Ser276) in paraffin-embedded Human lung carcinoma tissue using Phospho-NFkB p65 (Ser276) Polyclonal Antibody (Product # PA5-37718).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 NF-kB is a mediator of 5-Aza-2'deoxycytidine-induced HEXIM1 expression. (A) LNCaP and C4-2 cells were treated with 5-AzadC. Cell lysates were prepared and expression of phospho-NF-kB and NF-kB were analyzed by western blots. Represented are blots cut into strips prior to blotting to minimize the amounts of antibodies required. Figures are representative of at least 3 independent experiments. *P < 0.05 vs. Control. (B) C4-2 and LNCaP cells were treated with 2 uM of VE-822 or caffeine for 2 h, followed by 5-AzadC (5 µM) for 2 h. Cells were processed for ChIP analyses of the occupancy of NF-kB on the HEXIM1 promoter. Input DNA was used as normalization control. IgG control was used as a negative control. *P < 0.05 and **P < 0.01 vs. Control. (C) C4-2 and LNCaP cells were infected with lentiviruses expressing control or NF-kB shRNA, and selected with puromycin. Some cells were treated with 2 uM of VE-822 or caffeine for 2 h, followed by 5-AzadC (5 µM) for 2 h. Cell lysates were prepared and expression of indicated proteins were analyzed by western blot. Represented are blots cut into strips prior to blotting to minimize the amounts of antibodies required. Figures are representative of at least 3 independent experiments. (D) LNCaP and C4-2 cells were infected with control or NF-kB shRNA lentiviruses and selected with puromycin. Some cells were treated with 5-AzadC (5 muMu, 2 h). Cells were then processed for ChIP analyses of recruitment of CDK9 and NF-kB to th