Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC5078P - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse RelA/NFkB p65 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse RelA/NF kappa B p65 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
Q04206
- Isotype
- IgG
- Antibody clone number
- 532301
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.
Galle-Treger L, Sankaranarayanan I, Hurrell BP, Howard E, Lo R, Maazi H, Lewis G, Banie H, Epstein AL, Hu P, Rehan VK, Gilliland FD, Allayee H, Soroosh P, Sharpe AH, Akbari O
Nature communications 2019 Feb 12;10(1):713
Nature communications 2019 Feb 12;10(1):713
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.