Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [11]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 436700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p65 Monoclonal Antibody (572)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 572
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.
Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.
A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta.
Plastin 3 influences bone homeostasis through regulation of osteoclast activity.
Memory and PTPIP51--a new protein in hippocampus and cerebellum.
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.
Co-expression of β-arrestin1 and NF-кB is associated with cancer progression and poor prognosis in lung adenocarcinoma.
Targeting IκB proteins for HIV latency activation: the role of individual IκB and NF-κB proteins.
Tumor protein p63/nuclear factor κB feedback loop in regulation of cell death.
Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals.
Gu J, Li X, Zhao L, Yang Y, Xue C, Gao Y, Li J, Han Q, Sun Z, Bai C, Zhao RC
Cell death & disease 2021 Mar 17;12(4):291
Cell death & disease 2021 Mar 17;12(4):291
Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.
Lyu Q, Wawrzyniuk M, Rutten VPMG, van Eden W, Sijts AJAM, Broere F
International journal of molecular sciences 2020 Sep 4;21(18)
International journal of molecular sciences 2020 Sep 4;21(18)
A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta.
McClellan A, Evans R, Sze C, Kan S, Paterson Y, Guest D
Scientific reports 2019 Feb 26;9(1):2755
Scientific reports 2019 Feb 26;9(1):2755
Plastin 3 influences bone homeostasis through regulation of osteoclast activity.
Neugebauer J, Heilig J, Hosseinibarkooie S, Ross BC, Mendoza-Ferreira N, Nolte F, Peters M, Hölker I, Hupperich K, Tschanz T, Grysko V, Zaucke F, Niehoff A, Wirth B
Human molecular genetics 2018 Dec 15;27(24):4249-4262
Human molecular genetics 2018 Dec 15;27(24):4249-4262
Memory and PTPIP51--a new protein in hippocampus and cerebellum.
Brobeil A, Viard M, Petri MK, Steger K, Tag C, Wimmer M
Molecular and cellular neurosciences 2015 Jan;64:61-73
Molecular and cellular neurosciences 2015 Jan;64:61-73
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.
Shih MF, Pan KH, Cherng JY
International journal of molecular sciences 2015 Dec 4;16(12):28800-11
International journal of molecular sciences 2015 Dec 4;16(12):28800-11
Co-expression of β-arrestin1 and NF-кB is associated with cancer progression and poor prognosis in lung adenocarcinoma.
Yu J, Wang L, Zhang T, Shen H, Dong W, Ni Y, Du J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Aug;36(8):6551-8
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Aug;36(8):6551-8
Targeting IκB proteins for HIV latency activation: the role of individual IκB and NF-κB proteins.
Fernandez G, Zaikos TD, Khan SZ, Jacobi AM, Behlke MA, Zeichner SL
Journal of virology 2013 Apr;87(7):3966-78
Journal of virology 2013 Apr;87(7):3966-78
Tumor protein p63/nuclear factor κB feedback loop in regulation of cell death.
Sen T, Sen N, Huang Y, Sinha D, Luo ZG, Ratovitski EA, Sidransky D
The Journal of biological chemistry 2011 Dec 16;286(50):43204-13
The Journal of biological chemistry 2011 Dec 16;286(50):43204-13
Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals.
Brown A, Islam T, Adams R, Nerle S, Kamara M, Eger C, Marder K, Cohen B, Schifitto G, McArthur JC, Sacktor N, Pardo CA
Journal of neurovirology 2011 Aug;17(4):382-92
Journal of neurovirology 2011 Aug;17(4):382-92
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 was performed by loading 20 µg of HeLa (lane1), Jurkat (lane2), Raji (lane3), Daudi (lane4), THP-1 (lane5), Cos-7 (lane6) and MDCK (lane7) cell lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4ºC overnight. NFkB p65 was detected at ~ 65 kDa using NFkB p65 Mouse Monoclonal Antibody (Product # 436700) at 2-3 µg/mL in 5 % skim milk for 3 hours at room temperature on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). An extra band was observed at ~ 32 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NF-kappa-B (p65) Clone: 572 Product # 436700. Immunoflorescence of 4% PFA-fixed Hela cells (A) NF-kappa-B (P65) Clone: 572 Product # 436700 shown in green, Alexa Fluor® 488 secondary antibody. Nuclei are shown in blue with DAPI. (B) No primary antibody (negative control), Alexa Fluor® 488 secondary antibody, and Nuclei are shown in blue with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NF-kappa-B (p65) Clone: 572 (Product # 436700). Immunoprecipitation and Western Blot 1: 3T3 Western Blot, 2: Hela Western Blot, 3: IP negative control, 4: Heal + NF-kappa-B (P65) antibody IP positive control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NF-kappa-B (p65) Clone: 572 (Product # 436700). Immunoprecipitation and Western Blot 1: 3T3 Western Blot, 2: Hela Western Blot, 3: IP negative control, 4: Heal + NF-kappa-B (P65) antibody IP positive control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Effects of DEHP on phosphorylated p38 MAPK, phosphorylated ERK1/2, phosphorylated Akt, and NF-kappaB (p65) protein expression. VSMC ( n >= 3) were treated with DEHP (concentrations between 2 and 17.5 ppm) for 20 min (p38 MAPK, ERK1/2, and Akt) or 12 h (NF-kappaB) prior to protein extraction. Phosphorylated p38 MAPK, phosphorylated ERK1/2, phosphorylated Akt, and NF-kappaB (p65) were expressed by Western blotting. Statistics are shown for DEHP-treated cells * p < 0.05, ** p < 0.01, and *** p < 0.005, compared to the respective control groups.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 The effect of cell stress on NF-kappaB phosphorylation in LPS-stimulated 030D cells. The 030D cells were incubated with different concentrations (1.25 and 2.5 uM) of arsenite, or without, for 16 h, after which the cells were exposed to LPS and harvested at 5 min ( A , E ), 15 min ( B , F ), 30 min ( C , G ) and 60 min ( D , H ). Whole cell lysates were extracted and analyzed by Western Blotting. Control: untreated cells. Phosphorylated NF-kappaB and total NF-kappaB were detected with rabbit monoclonal anti-phospho-NF-kappaB p65 and HRP-labeled swine-anti rabbit IgG, and mouse monoclonal anti- NF-kappaB p65 and HRP-labeled rabbit anti-mouse IgG, respectively. The densitometry of the protein bands was scanned and quantitated with Image lab TM software 6.0.1 ( E - H ). The total NF-kappaB levels were used as an internal control. Data are shown as the mean +- SD and are representative of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001, vs. LPS alone group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 The effect of a deficiency of inducible Hsp70 on pro-inflammatory cytokine expression and NF-kappaB phosphorylation. Hsp70 knockout 030D cells were treated with different concentrations (1.25, 2.5 or 5 uM) of arsenite, or without, for 16 h, and then exposed to LPS. The cells were harvested after 6 h ( A - C ) of LPS exposure. qPCR was performed to detect the expression of IL-6 ( A ), IL-1beta ( B ) and TNF-alpha ( C ). For Western Blotting, the cells were harvested after 5 ( D , H ), 15 ( E , I ), 30 ( F , J ) and 60 ( G , K ) min of LPS exposure. Western Blotting was performed to detect levels of phosphorylated NF-kappaB and total NF-kappaB at certain time points. The densitometry of the protein bands was scanned and quantitated with Image lab TM software 6.0.1. ( H - K ). The total NF-kappaB levels were used as an internal control. Data are shown as the mean +- SD and are representative of three independent experiments. * p < 0.05, ** p < 0.01 vs. LPS alone group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 PKM2 and P65 in the nucleus were simultaneously upregulated during the differentiation of MSCs into CAFs induced by gastric cancer exosomes. A , B The protein-level changes of glycolysis enzymes (Hexokinase I, Hexokinase II, PKM1/2, PKM2, LDHA, PFKP, and PDH) during the differentiation of MSCs into CAFs induced by AGS exosomes ( A ) or GC803 exosomes ( B ) were evaluated by western blot. C Confocal microscopy detected PKM2 and P65 expression in the nucleus during the differentiation of MSCs into CAFs induced by AGS exosomes. Blue: Nucleus (Hoechst 33342). Red: P65 (rhodamine). Green: PKM2 (Alexa Fluor 488. D , E Western blotting analysis detected PKM2 and P65 changes in the nucleus.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 The interaction between PKM2 and P65. A The PKM2 promoter region had a binding box of P65. B Quantitative PCR analysis detected the chromatin-protein complex captured by CHIP P65 antibody containing the PKM2 promoter region sequence. * P < 0.05. C Western blotting analysis detected the whole-cell lysate protein captured by Co-IP P65 antibody containing PKM2 protein. D Western blotting analysis detected cell lysate protein captured by Co-IP PKM2 antibody containing P65 protein.