LS-C497320

antibody from LSBio

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C497320

Provider

LSBio

Product name

RELA / NFKB p65 Antibody LS-C497320

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using RELA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.