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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB1962 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1962, RRID:AB_1579689
- Product name
- RELA (phospho S536) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S536 of human RELA.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Requirement for caspase-8 in NF-kappaB activation by antigen receptor.
Chromatin-dependent E1A activity modulates NF-kappaB RelA-mediated repression of glucocorticoid receptor-dependent transcription.
Function of polo-like kinase 3 in NF-kappaB-mediated proapoptotic response.
Su H, Bidère N, Zheng L, Cubre A, Sakai K, Dale J, Salmena L, Hakem R, Straus S, Lenardo M
Science (New York, N.Y.) 2005 Mar 4;307(5714):1465-8
Science (New York, N.Y.) 2005 Mar 4;307(5714):1465-8
Chromatin-dependent E1A activity modulates NF-kappaB RelA-mediated repression of glucocorticoid receptor-dependent transcription.
Burkhart BA, Hebbar PB, Trotter KW, Archer TK
The Journal of biological chemistry 2005 Feb 25;280(8):6349-58
The Journal of biological chemistry 2005 Feb 25;280(8):6349-58
Function of polo-like kinase 3 in NF-kappaB-mediated proapoptotic response.
Li Z, Niu J, Uwagawa T, Peng B, Chiao PJ
The Journal of biological chemistry 2005 Apr 29;280(17):16843-50
The Journal of biological chemistry 2005 Apr 29;280(17):16843-50
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RELA (phospho S536) polyclonal antibody (Cat # PAB1962) in Ramos cell line lysate (35 ug/lane). Phospho-RELA (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RELA (phospho S536) polyclonal antibody (Cat # PAB1962) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
