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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- PAB10290 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10290, RRID:AB_1710299
- Product name
- RELA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RELA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular mechanisms of NF-kappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors.
Hormonal regulation of the NF-kappaB signaling pathway.
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus.
Zhang G, Ghosh S
Journal of endotoxin research 2000;6(6):453-7
Journal of endotoxin research 2000;6(6):453-7
Hormonal regulation of the NF-kappaB signaling pathway.
Delfino F, Walker WH
Molecular and cellular endocrinology 1999 Nov 25;157(1-2):1-9
Molecular and cellular endocrinology 1999 Nov 25;157(1-2):1-9
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Unlap MT, Jope RS
Brain research. Molecular brain research 1997 Apr;45(1):83-9
Brain research. Molecular brain research 1997 Apr;45(1):83-9
Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus.
Zabel U, Henkel T, Silva MS, Baeuerle PA
The EMBO journal 1993 Jan;12(1):201-11
The EMBO journal 1993 Jan;12(1):201-11
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in TNF stimulated DU 145 cells. Image shown is at a 1 : 400 magnification.Tissue was fixed and prepared as above.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in (Lane 1) control DU 145 cells and (Lane 2) TNF stimulated DU 145 cells.Although DU 145 show relatively high basal levels of nuclear RELA staining, significant enhancement of nuclear staining is seen in pB as evidence of translocation and availability of the NLS to be bound by the antibody.Cultured cells shown above were formalin-fixed.
- Validation comment
- Immunocytochemistry
