PAB10337

antibody from Abnova Corporation

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10337

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10337, RRID:AB_1710349

Product name

RELA (phospho S529) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.

Antigen sequence

PNGLLpSGDEDFC


Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.

Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7

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Series introduction: the transcription factor NF-kappaB and human disease.

Baldwin AS Jr
The Journal of clinical investigation 2001 Jan;107(1):3-6

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Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.

Baldwin AS
The Journal of clinical investigation 2001 Feb;107(3):241-6

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TNF Induces phosphorylation of RELA in KBM-5 cells.Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using RELA (phospho S529) polyclonal antibody (Cat # PAB10337).Anti-beta actin staining confirms loading of equivalent amounts of protein.HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.Data contributed by Aggarwal BB, personal communication.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Anti-pS529 shows phospho RELA staining in carcinoma cells.Immunoblot of total protein lysates from various human head and neck tumors shows phospho RELA staining in tumor cell lines using p65.Lanes 1 - 6 contain protein lysates from human squamal carcinoma cell lines.Lane 7 is a protein lysate from a primary culture of human keratinocytes.Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma).Lane 9 contains lysate from EGF-induced human derived A-431 cells.Lane 10 (not shown) contains a molecular weight standard.Concurrent staining with anti-beta microtubulin (not shown) was used to confirmequal protein loading in all lanes.HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.Data contributed by Yu, M., NIH, personal communication.