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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-67284 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal RelA/NFkB p65 NLS Antibody
- Antibody type
- Monoclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RelA/NFkB p65 NLS Antibody (Rabbit Polyclonal Antibody) [NBP2-67284] - Analysis of NF-kB p65 on different cell lysates using anti-NF-kB p65 antibody at 1/1000 dilution. Positive control: Lane1: Hela Lane2:A549 Lane3: PC12 Lane 4: Mouse embryonic Lane5: NIH/3T3 stem cell
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody (Rabbit Polyclonal Antibody) [NBP2-67284] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- NF-kB p65 antibody. Counter stained with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody (Rabbit Polyclonal Antibody) [NBP2-67284] - Analysis of paraffin-embedded human lung carcinoma tissue using anti- NF-kB p65 antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RelA/NFkB p65 NLS Antibody (Rabbit Polyclonal Antibody) [NBP2-67284] - Analysis of Hela cells with NF-kB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
