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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [33]
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- Product number
- 100-4165S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-4165S, RRID:AB_11183750
- Product name
- Anti-NFKB p65 (Rel A) (RABBIT) Antibody - 100-4165S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references Metabolic Control of Sensory Neuron Survival by the p75 Neurotrophin Receptor in Schwann Cells.
NFκB signaling in alveolar rhabdomyosarcoma.
Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice.
Role of physical exercise on hepatic insulin, glucocorticoid and inflammatory signaling pathways in an animal model of non-alcoholic steatohepatitis.
Chitosan oligosaccharides attenuate ocular inflammation in rats with experimental autoimmune anterior uveitis.
The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia.
Rasagiline and selegiline, inhibitors of type B monoamine oxidase, induce type A monoamine oxidase in human SH-SY5Y cells.
Depletion of the receptor for advanced glycation end products (RAGE) sensitizes towards apoptosis via p53 and p73 posttranslational regulation.
Targeting activation of specific NF-κB subunits prevents stress-dependent atherothrombotic gene expression.
Endometrial receptivity defects and impaired implantation in diabetic NOD mice.
Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies.
Activation of brain endothelium by soluble aggregates of the amyloid-β protein involves nuclear factor-κB.
Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation.
CXCL5 promotes prostate cancer progression.
Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype.
Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms.
NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages.
CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro.
Expression and deoxyribonucleic acid-binding activity of the nuclear factor kappaB family in the human myometrium during pregnancy and labor.
NF-kappaB inducible genes BCL-X and cyclin E promote immature B-cell proliferation and survival.
N-Propargyl-1 (R)-aminoindan, rasagiline, increases glial cell line-derived neurotrophic factor (GDNF) in neuroblastoma SH-SY5Y cells through activation of NF-kappaB transcription factor.
Divergent C-terminal transactivation domains of Rel/NF-kappa B proteins are critical determinants of their oncogenic potential in lymphocytes.
Efficient replication by herpes simplex virus type 1 involves activation of the IkappaB kinase-IkappaB-p65 pathway.
Cyclin E and Bcl-xL cooperatively induce cell cycle progression in c-Rel-/- B cells.
Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation.
Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype.
Opposing regulation of choline deficiency-induced apoptosis by p53 and nuclear factor kappaB.
Propanil inhibits tumor necrosis factor-alpha production by reducing nuclear levels of the transcription factor nuclear factor-kappab in the macrophage cell line ic-21.
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity.
Regulation of human leukocyte antigen expression in human conjunctival epithelium.
Interaction of tumor necrosis factor receptor-associated factor signaling proteins with the latent membrane protein 1 PXQXT motif is essential for induction of epidermal growth factor receptor expression.
The NPC derived C15 LMP1 protein confers enhanced activation of NF-kappa B and induction of the EGFR in epithelial cells.
Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation.
Follis RM, Tep C, Genaro-Mattos TC, Kim ML, Ryu JC, Morrison VE, Chan JR, Porter N, Carter BD, Yoon SO
The Journal of neuroscience : the official journal of the Society for Neuroscience 2021 Oct 20;41(42):8710-8724
The Journal of neuroscience : the official journal of the Society for Neuroscience 2021 Oct 20;41(42):8710-8724
NFκB signaling in alveolar rhabdomyosarcoma.
Cleary MM, Mansoor A, Settelmeyer T, Ijiri Y, Ladner KJ, Svalina MN, Rubin BP, Guttridge DC, Keller C
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice.
Ryu S, Han HM, Song PI, Armstrong CA, Park Y
PloS one 2015;10(7):e0132619
PloS one 2015;10(7):e0132619
Role of physical exercise on hepatic insulin, glucocorticoid and inflammatory signaling pathways in an animal model of non-alcoholic steatohepatitis.
Passos E, Pereira CD, Gonçalves IO, Rocha-Rodrigues S, Silva N, Guimarães JT, Neves D, Ascensão A, Magalhães J, Martins MJ
Life sciences 2015 Feb 15;123:51-60
Life sciences 2015 Feb 15;123:51-60
Chitosan oligosaccharides attenuate ocular inflammation in rats with experimental autoimmune anterior uveitis.
Fang IM, Yang CH, Yang CM
Mediators of inflammation 2014;2014:827847
Mediators of inflammation 2014;2014:827847
The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia.
Hariri F, Arguello M, Volpon L, Culjkovic-Kraljacic B, Nielsen TH, Hiscott J, Mann KK, Borden KL
Leukemia 2013 Oct;27(10):2047-55
Leukemia 2013 Oct;27(10):2047-55
Rasagiline and selegiline, inhibitors of type B monoamine oxidase, induce type A monoamine oxidase in human SH-SY5Y cells.
Inaba-Hasegawa K, Akao Y, Maruyama W, Naoi M
Journal of neural transmission (Vienna, Austria : 1996) 2013 Mar;120(3):435-44
Journal of neural transmission (Vienna, Austria : 1996) 2013 Mar;120(3):435-44
Depletion of the receptor for advanced glycation end products (RAGE) sensitizes towards apoptosis via p53 and p73 posttranslational regulation.
Brune M, Müller M, Melino G, Bierhaus A, Schilling T, Nawroth PP
Oncogene 2013 Mar 14;32(11):1460-8
Oncogene 2013 Mar 14;32(11):1460-8
Targeting activation of specific NF-κB subunits prevents stress-dependent atherothrombotic gene expression.
Djuric Z, Kashif M, Fleming T, Muhammad S, Piel D, von Bauer R, Bea F, Herzig S, Zeier M, Pizzi M, Isermann B, Hecker M, Schwaninger M, Bierhaus A, Nawroth PP
Molecular medicine (Cambridge, Mass.) 2012 Dec 20;18(1):1375-86
Molecular medicine (Cambridge, Mass.) 2012 Dec 20;18(1):1375-86
Endometrial receptivity defects and impaired implantation in diabetic NOD mice.
Albaghdadi AJ, Kan FW
Biology of reproduction 2012 Aug;87(2):30
Biology of reproduction 2012 Aug;87(2):30
Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies.
Herkenham M, Rathore P, Brown P, Listwak SJ
Journal of neuroinflammation 2011 Oct 14;8:141
Journal of neuroinflammation 2011 Oct 14;8:141
Activation of brain endothelium by soluble aggregates of the amyloid-β protein involves nuclear factor-κB.
Gonzalez-Velasquez F, Reed JW, Fuseler JW, Matherly EE, Kotarek JA, Soto-Ortega DD, Moss MA
Current Alzheimer research 2011 Feb;8(1):81-94
Current Alzheimer research 2011 Feb;8(1):81-94
Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation.
Limpert AS, Carter BD
The Journal of biological chemistry 2010 May 28;285(22):16614-22
The Journal of biological chemistry 2010 May 28;285(22):16614-22
CXCL5 promotes prostate cancer progression.
Begley LA, Kasina S, Mehra R, Adsule S, Admon AJ, Lonigro RJ, Chinnaiyan AM, Macoska JA
Neoplasia (New York, N.Y.) 2008 Mar;10(3):244-54
Neoplasia (New York, N.Y.) 2008 Mar;10(3):244-54
Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype.
Yoon C, Korade Z, Carter BD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 2;28(14):3738-46
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 2;28(14):3738-46
Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms.
Lou H, Kaplowitz N
The Journal of biological chemistry 2007 Oct 5;282(40):29470-81
The Journal of biological chemistry 2007 Oct 5;282(40):29470-81
NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages.
Cao S, Zhang X, Edwards JP, Mosser DM
The Journal of biological chemistry 2006 Sep 8;281(36):26041-50
The Journal of biological chemistry 2006 Sep 8;281(36):26041-50
CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro.
Begley L, Monteleon C, Shah RB, Macdonald JW, Macoska JA
Aging cell 2005 Dec;4(6):291-8
Aging cell 2005 Dec;4(6):291-8
Expression and deoxyribonucleic acid-binding activity of the nuclear factor kappaB family in the human myometrium during pregnancy and labor.
Chapman NR, Europe-Finner GN, Robson SC
The Journal of clinical endocrinology and metabolism 2004 Nov;89(11):5683-93
The Journal of clinical endocrinology and metabolism 2004 Nov;89(11):5683-93
NF-kappaB inducible genes BCL-X and cyclin E promote immature B-cell proliferation and survival.
Feng B, Cheng S, Hsia CY, King LB, Monroe JG, Liou HC
Cellular immunology 2004 Nov-Dec;232(1-2):9-20
Cellular immunology 2004 Nov-Dec;232(1-2):9-20
N-Propargyl-1 (R)-aminoindan, rasagiline, increases glial cell line-derived neurotrophic factor (GDNF) in neuroblastoma SH-SY5Y cells through activation of NF-kappaB transcription factor.
Maruyama W, Nitta A, Shamoto-Nagai M, Hirata Y, Akao Y, Yodim M, Furukawa S, Nabeshima T, Naoi M
Neurochemistry international 2004 May;44(6):393-400
Neurochemistry international 2004 May;44(6):393-400
Divergent C-terminal transactivation domains of Rel/NF-kappa B proteins are critical determinants of their oncogenic potential in lymphocytes.
Fan Y, Rayet B, Gélinas C
Oncogene 2004 Feb 5;23(5):1030-42
Oncogene 2004 Feb 5;23(5):1030-42
Efficient replication by herpes simplex virus type 1 involves activation of the IkappaB kinase-IkappaB-p65 pathway.
Gregory D, Hargett D, Holmes D, Money E, Bachenheimer SL
Journal of virology 2004 Dec;78(24):13582-90
Journal of virology 2004 Dec;78(24):13582-90
Cyclin E and Bcl-xL cooperatively induce cell cycle progression in c-Rel-/- B cells.
Cheng S, Hsia CY, Leone G, Liou HC
Oncogene 2003 Nov 20;22(52):8472-86
Oncogene 2003 Nov 20;22(52):8472-86
Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation.
Haller D, Holt L, Kim SC, Schwabe RF, Sartor RB, Jobin C
The Journal of biological chemistry 2003 Jun 27;278(26):23851-60
The Journal of biological chemistry 2003 Jun 27;278(26):23851-60
Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype.
Poser I, Golob M, Buettner R, Bosserhoff AK
Molecular and cellular biology 2003 Apr;23(8):2991-8
Molecular and cellular biology 2003 Apr;23(8):2991-8
Opposing regulation of choline deficiency-induced apoptosis by p53 and nuclear factor kappaB.
Holmes-McNary MQ, Baldwin AS Jr, Zeisel SH
The Journal of biological chemistry 2001 Nov 2;276(44):41197-204
The Journal of biological chemistry 2001 Nov 2;276(44):41197-204
Propanil inhibits tumor necrosis factor-alpha production by reducing nuclear levels of the transcription factor nuclear factor-kappab in the macrophage cell line ic-21.
Frost LL, Neeley YX, Schafer R, Gibson LF, Barnett JB
Toxicology and applied pharmacology 2001 May 1;172(3):186-93
Toxicology and applied pharmacology 2001 May 1;172(3):186-93
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity.
Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB
Journal of immunology (Baltimore, Md. : 1950) 1999 Sep 15;163(6):3474-83
Journal of immunology (Baltimore, Md. : 1950) 1999 Sep 15;163(6):3474-83
Regulation of human leukocyte antigen expression in human conjunctival epithelium.
Tsubota K, Fukagawa K, Fujihara T, Shimmura S, Saito I, Saito K, Takeuchi T
Investigative ophthalmology & visual science 1999 Jan;40(1):28-34
Investigative ophthalmology & visual science 1999 Jan;40(1):28-34
Interaction of tumor necrosis factor receptor-associated factor signaling proteins with the latent membrane protein 1 PXQXT motif is essential for induction of epidermal growth factor receptor expression.
Miller WE, Cheshire JL, Raab-Traub N
Molecular and cellular biology 1998 May;18(5):2835-44
Molecular and cellular biology 1998 May;18(5):2835-44
The NPC derived C15 LMP1 protein confers enhanced activation of NF-kappa B and induction of the EGFR in epithelial cells.
Miller WE, Cheshire JL, Baldwin AS Jr, Raab-Traub N
Oncogene 1998 Apr 9;16(14):1869-77
Oncogene 1998 Apr 9;16(14):1869-77
Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation.
Cheshire JL, Baldwin AS Jr
Molecular and cellular biology 1997 Nov;17(11):6746-54
Molecular and cellular biology 1997 Nov;17(11):6746-54
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell extract. Lane 2: HeLa cell extract. Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None.
- Validation comment
- Western Blot
