MA5-51360

antibody from Invitrogen Antibodies

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-51360

Provider

Invitrogen Antibodies

Product name

NFkB p65 Recombinant Rabbit Monoclonal Antibody (6D6R8)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: LGALLGNSTD PAVFTDLASV DNSEFQQLLN QGIPVAPHTT EPMLMEYPEA ITRLVTGAQR PPDPAPAPLG APGLPNGLLS GDEDFSSIAD MDFSALLSQI SS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

6D6R8

Vial size

100 μL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFkB p65 in extracts from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa cells. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:10,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFkB p65 in extracts of various cell lines. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:10,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry (confocal imaging) analysis of NFkB p65 in HeLa cells. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:100 (Red). The cells were counterstained with Monomethyl-HistoneH3-K4 Rabbit mAb (dilution 1:300) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFkB p65 in paraffin-embedded human colon carcinoma. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFkB p65 in paraffin-embedded human liver. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NFkB p65 in paraffin-embedded human liver. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP assay of NFkB p65 in HT-1080 cells treated by TNF alpha (20 ng/mL) at 37°C for 30 minutes. Samples were incubated with NFkB p65 Recombinant monoclonal antibody (Product # MA5-51360) with rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.