MA5-15563

antibody from Invitrogen Antibodies

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15563

Provider

Invitrogen Antibodies

Product name

NFkB p65 Monoclonal Antibody (6H7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15563 targets NF-κ-B p65 in indirect ELISA, WB applications and shows reactivity with Human and mouse samples. The MA5-15563 immunogen is purified recombinant fragment of human NF-κ-B p65 expressed in E. Coli. MA5-15563 detects NF-κ-B p65 which has a predicted molecular weight of approximately 65kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

6H7

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

Sakowicz A, Bralewska M, Pietrucha T, Figueras F, Habrowska-Górczyńska DE, Piastowska-Ciesielska AW, Gach A, Sakowicz B, Rybak-Krzyszkowska M, Huras H, Grzesiak M, Biesiada L
International journal of molecular sciences 2021 Sep 22;22(19)

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The anesthetic sevoflurane induces tau trafficking from neurons to microglia.

Dong Y, Liang F, Huang L, Fang F, Yang G, Tanzi RE, Zhang Y, Quan Q, Xie Z
Communications biology 2021 May 12;4(1):560

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Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.

Verma S, Mohapatra G, Ahmad SM, Rana S, Jain S, Khalsa JK, Srikanth CV
Molecular and cellular biology 2015 Sep 1;35(17):2932-46

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-Kappa-B p65 using NF-Kappa-B p65 monoclonal antibody (Product # MA5-15563) in Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Western blot results. ( a ) inhibition of p53 by its targeted siRNA; Western blot results for the cytoplasmic fraction, ( b ) the differences in relative level of pNFkB Ser536 to Histone H3 in nucleus of HTR8/SVneo cells. Cells were cultured for 96 h in hypoxic or normoxic conditions with medium containing 1% serum from preeclamptic or normotensive women. For the first 48 h, the cells were incubated only with medium containing the maternal serum. After 48 h and 72 h, the cell medium was supplemented with the following factors: none i.e., cells without siRNA (1), non-targeting siRNA (2) and targeted siRNA against mRNA coding for p53 (3). The relative level of pNFkB Ser536 to Histone H3 in the nucleus was analyzed. Values are presented as mean +- SEM; ( n = 3experiments). The difference in pNFkB Ser536 level between cells stimulated by negative and targeted siRNA was calculated by the Student's t -test; ## p < 0.01. The difference in pNFkB Ser536 level between the four culture types (i.e., oxygen tension and serum type) which were not stimulated with siRNA were analyzed by ANOVA with the Bonferroni post hoc test; ** p < 0.01, *** p < 0.001. ( c ) Western blot results for nuclear fraction containing pNFkB Ser536 and Histone H3, as a loading control.