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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [10]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-27617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p65 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, K562, THP-1, HL-60, human NFkB p65-transfected 293T.
- Concentration
- 1.37 mg/mL
Submitted references Diacerein counteracts acetaminophen-induced hepatotoxicity in mice via targeting NLRP3/caspase-1/IL-1β and IL-4/MCP-1 signaling pathways.
Elshal M, Abdelmageed ME
Archives of pharmacal research 2022 Mar;45(3):142-158
Archives of pharmacal research 2022 Mar;45(3):142-158
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 using Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-27617) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p65 using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NFkB p65 polyclonal antibody (Product # PA5-27617) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p65 in Non-transfected (-) and NFkB p65 -transfected (+, inculding GFP-tag) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with NFkB p65 Polyclonal antibody (Product # PA5-27617) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-27617) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- J-Lat Full Length cell lysate 40 µg was loaded on 4-15% gel and transferred to 0.45 µm PVDF Membrane. NFkB p65 Polyclonal Antibody (Product # PA5-27617) was diluted at 1:500 in 1xTBST buffer with 5% non fat milk and incubated overnight at 4C. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at 21C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- J-Lat Full Length cell lysate 40 µg was loaded on 4-15% gel and transferred to 0.45 µm PVDF Membrane. NFkB p65 Polyclonal Antibody (Product # PA5-27617) was diluted at 1:500 in 1xTBST buffer with 5% non fat milk and incubated overnight at 4C. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at 21C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-27617) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-27617) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFkB p65 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFkB p65 Polyclonal Antibody (Product # PA5-27617) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NFkB p65 Polyclonal Antibody (Product # PA5-27617). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 Polyclonal Antibody (Product # PA5-27617) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFkB p65 in paraformaldehyde-fixed U2OS cells using a NFkB p65 polyclonal antibody (Product # PA5-27617) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NFkB p65 stained by NFkB p65 Polyclonal Antibody (Product # PA5-27617) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFkB p65 stained by NFkB p65 Polyclonal Antibody (Product # PA5-27617) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NFkB p65 Polyclonal Antibody (Product # PA5-27617) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
