Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX30886 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30886, RRID:AB_10618077
- Product name
- NFKB p65 (phospho Ser536) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Submitted references Morphoproteomics identifies constitutive activation of the mTORC2/Akt and NF-κB pathways and expressions of IGF-1R, Sirt1, COX-2, and FASN in peripheral T-cell lymphomas: pathogenetic implications and therapeutic options.
Quesada AE, Nguyen ND, Rios A, Brown RE
International journal of clinical and experimental pathology 2014;7(12):8732-9
International journal of clinical and experimental pathology 2014;7(12):8732-9
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-NFKBp65-S536 Antibody (GTX30886) in human TNF alpha activated Hela cell line lysates. Phospho-RPS6KB1 (arrow) was detected using the purified PAb. Lane 1: without TNF alpha Lane 2: 10ng/ml TNF alpha, 5min
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary NFKB p65 antibody (GTX30886), which was peroxidase-conjμgated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry.HC = hepatocarcinoma