GTX630198

antibody from GeneTex

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Immunocytochemistry

Antibody data

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Validation data

MTOR protein structure - GTX630198 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41512

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: mTOR antibody [GT649] detects mTOR protein by western blot analysis.A Jurkat BRaji whole cell lysate/extractC NCI-H929 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT649] (GTX630198) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: mTOR antibody [GT649] detects mTOR protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT649] (GTX630198) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500.