GTX630199

antibody from GeneTex

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX630199

Provider

GeneTex

Product name

mTOR antibody [GT6611]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] (GTX630199) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41512

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A Jurkat whole cell lysate/extract BRaji C NCI-H929 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT6611] (GTX630199) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR antibody [GT6611] detects mTOR protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extractD. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEmTOR antibody [GT6611] (GTX630199) dilution: 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] (GTX630199) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR antibody [GT6611] detects mTOR protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: mTOR protein stained by mTOR antibody [GT6611] (GTX630199) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.