AP3433a

antibody from Abgent

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3433a

Provider

Abgent

Proper citation

Abgent Cat#AP3433a, RRID:AB_1278043

Product name

Phospho-FRAP1(T2446) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.45 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway.

Pillai VB, Sundaresan NR, Kim G, Gupta M, Rajamohan SB, Pillai JB, Samant S, Ravindra PV, Isbatan A, Gupta MP
The Journal of biological chemistry 2010 Jan 29;285(5):3133-44

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Dot blot analysis of anti-FRAP1-pT2446 Phospho-specific Pab (Cat.AP3433a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Primary Ab dilution

1:500