GTX101558

antibody from GeneTex

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Antibody data

Submit

Validation data

MTOR protein structure - GTX101558 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 39721

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE GTX101558 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101558) diluted at a dilution of 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Mouse tissue extracts (50 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:500.