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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- IC1537P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TOR/mTOR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TOR in direct ELISAs. Detects human and mouse TOR in flow cytometry.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TOR in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Human IL-2 (Catalog # 202-IL) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TOR in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with Anti-CD3, Anti-CD28, Recombinant Human TGF-beta 1 (Catalog # 240-B), and Recombinant Mouse IL-2 (Catalog # 402-ML) were stained with Rat Anti-Human/Mouse TOR PE-conjugated Monoclonal Antibody (Catalog # IC1537P) and Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
