701483

antibody from Invitrogen Antibodies

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

701483

Provider

Invitrogen Antibodies

Product name

mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

7H13L18

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Polycystin-1 affects cancer cell behaviour and interacts with mTOR and Jak signalling pathways in cancer cell lines.

Papavassiliou KA, Zoi I, Gargalionis AN, Koutsilieris M
Journal of cellular and molecular medicine 2019 Sep;23(9):6215-6227

---

mTOR kinase domain phosphorylation promotes mTORC1 signaling, cell growth, and cell cycle progression.

Ekim B, Magnuson B, Acosta-Jaquez HA, Keller JA, Feener EP, Fingar DC
Molecular and cellular biology 2011 Jul;31(14):2787-801

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mTOR in whole cell extracts from serum-starved HEK293 using a mTOR recombinant rabbit monoclonal antibody (Product # 701483) at a dilution of 2 µg/mL. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~289kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mTOR in HeLa cells using a mTOR recombinant rabbit monoclonal antibody (Product # 701483) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear localization of mTOR.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of mTOR in HeLa cells using a mTOR recombinant rabbit monoclonal antibody (Product # 701483). Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (right peak) compared to an isotype control (middle peak, black) and a control without primary antibody (left peak, blue).