GTX132803

antibody from GeneTex

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX132803

Provider

GeneTex

Product name

mTOR (phospho Ser2448) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

The Inhibition of microRNA-128 on IGF-1-Activating mTOR Signaling Involves in Temozolomide-Induced Glioma Cell Apoptotic Death.

Chen PH, Cheng CH, Shih CM, Ho KH, Lin CW, Lee CC, Liu AJ, Chang CK, Chen KC
PloS one 2016;11(11):e0167096

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) 293T whole cell extracts (60 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) NIH-3T3 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

mTOR (phospho Ser2448) antibody detects mTOR (phospho Ser2448) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded human lung cancer.mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500.