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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- R1472P - Provider product page
- Provider
- OriGene
- Product name
- MTOR pSer2448 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- MTOR pSer2448 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MTOR
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.88 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoblotting. mTOR antibody (pSer2448) is shown to detect a 250kDa band (indicated) corresponding to phosphorylated Human mTOR present in a 293Twhole cell lysates. Cells were serumstarved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in Lane 1, whereas cells in Lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1/2000 dilution of HRP Goat anti-Rabbit IgG for visualization.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: pSer2448 mTOR antibody staining of Formalin-Fixed, Paraffin Embedded Sections. This antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-Human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. The image shows localization of the antibody as the precipitated Red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Seattle, WA.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Metabolic and autocrine regulation of the mTOR pathway by b-cells.
- Validation comment
- Assay
