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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- AP09299PU-N - Provider product page
- Provider
- OriGene
- Product name
- MTOR (2440-2457) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- MTOR (2440-2457) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MTOR
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.40 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-mTOR antibody shows detection of a band ~245 kDa corresponding to human mTor (arrowhead). Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Metabolic and autocrine regulation of the mTOR pathway by beta-cells.
- Validation comment
- Assay
