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Learn28130002-0.1mg
antibody from Novus Biologicals
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- 28130002-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TOR/mTOR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product is specific for Human FRAP1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TOR/mTOR Antibody [28130002]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TOR/mTOR Antibody [28130002] - Several commercially available antibodies which were generated using short peptides failed to isolate the FRAP1 protein complexes. SDI's FRAP1(TOR/mTOR) antibody, 2813.00.02, was generated using in vivo expressed immunogen of 100 amino acids. It was used to pull down protein complexes through immunoprecipitation. It demonstrated that Rictor and Raptor formed the distinct complexes with FRAP (mTOR). The immunoprecipitation conditions were described in Sarbassov et al (Phosphorylation and regulation of Akt/PKB by the Rictor-mTOR complex, Science 307:1098-1101, 2005, supporting online material, material and methods). FRAP1 was detected in Western blot using FRAP1 antibody at 1:4000 dilution. The experiments were performed using MDA MB-435 human cancer cell line and NF2 was used as control.
