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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1988 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1988, RRID:AB_1574960
- Product name
- MTOR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FRAP1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activation of the PTEN/mTOR/STAT3 pathway in breast cancer stem-like cells is required for viability and maintenance.
Metformin inhibits mammalian target of rapamycin-dependent translation initiation in breast cancer cells.
Zhou J, Wulfkuhle J, Zhang H, Gu P, Yang Y, Deng J, Margolick JB, Liotta LA, Petricoin E 3rd, Zhang Y
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 9;104(41):16158-63
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 9;104(41):16158-63
Metformin inhibits mammalian target of rapamycin-dependent translation initiation in breast cancer cells.
Dowling RJ, Zakikhani M, Fantus IG, Pollak M, Sonenberg N
Cancer research 2007 Nov 15;67(22):10804-12
Cancer research 2007 Nov 15;67(22):10804-12
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MTOR (arrow) using rabbit polyclonal MTOR polyclonal antibody (Cat # PAB1988). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MTOR gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with MTOR polyclonal antibody (Cat # PAB1988) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
