14-2190-80
antibody from Invitrogen Antibodies
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 14-2190-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mTOR Monoclonal Antibody (F11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This F11 monoclonal antibody reacts with human, mouse, and rat mammalian target of rapamycin (mTOR). This 289-kDa serine/threonine kinase is activated via phosphorylation at Ser2448 by the PI3K/Akt signaling pathway in response to growth factors, energy, nutrients, and stress. mTOR is highly conserved amongst eukaryotes and constitutively expressed in immune (e.g. T, B, NK, dendritic cells, mast cells, and neutrophils) and non-immune tissues (e.g. heart). mTOR exists as a member of two distinct complexes, mTORC1 and mTORC2, which have differing sensitivities to rapamycin and phosphorylation targets. Rapamycin inhibits mTOR activity by binding FKBP12, which causes dissociation of mTORC1 and mTORC2. This molecule plays a critical role in cell metabolism, survival, dendritic cell maturation and activation, and protein synthesis. Deregulation of mTOR has been implicated in a variety of cancers. Studies have also suggested a role for mTOR in Foxp3 expression and regulatory T cell differentiation.
- Antibody clone number
- F11
- Concentration
- 0.5 mg/mL
Submitted references Immunoregulatory functions of mTOR inhibition.
mTOR: taking cues from the immune microenvironment.
Generation and characterization of monoclonal antibodies to mTOR kinase.
Thomson AW, Turnquist HR, Raimondi G
Nature reviews. Immunology 2009 May;9(5):324-37
Nature reviews. Immunology 2009 May;9(5):324-37
mTOR: taking cues from the immune microenvironment.
Delgoffe GM, Powell JD
Immunology 2009 Aug;127(4):459-65
Immunology 2009 Aug;127(4):459-65
Generation and characterization of monoclonal antibodies to mTOR kinase.
Nemazanyy I, Breus O, Gout I, Filonenko V, Panasyuk G
Hybridoma (2005) 2008 Oct;27(5):395-9
Hybridoma (2005) 2008 Oct;27(5):395-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoblotting of reduced cell lysates prepared from Jurkat cell line with 5 µg/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.