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Learn710216
antibody from Invitrogen Antibodies
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 710216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-mTOR (Ser2448) Recombinant Polyclonal Antibody (8HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells.
Zhao Z, Ma J, Mao Y, Dong L, Li S, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 27;24:6851-6860
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 27;24:6851-6860
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-mTOR pSer2448 in whole cell extracts of serum-starved A431 treated with 100 ng/mL of EGF for 5 min (lanes 1 and 2) using a Phospho-mTOR pSer2448 Recombinant Rabbit Polyclonal Antibody (Product # 710216) at a dilution of 1 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Samples were detected using chemiluminescence (ECL). Results show a band at ~289 kDa.
