M00003

antibody from Boster Biological Technology

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Provider product page for M00003

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [27]
Comments [0]
Validations
Western blot [1]

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Product number: M00003 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-mTOR/Tor Rabbit Monoclonal Antibody
Antibody type: Monoclonal
Description: Monoclonal antibody for TOR/MTOR detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry. Reactive species: Human, Mouse, Rat TOR/MTOR information: Molecular Weight: 288892 MW; Subcellular Localization: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137); Tissue Specificity: Expressed in numerous tissues, with highest levels in testis.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Antibody clone number: CBD-13
Vial size: 100ug/vial
Storage: At -20°C for one year. Avoid repeated freezing and thawing.

MTOR protein structure - M00003 shown in red.

Submitted references Cantharidin-induced toxic injury, oxidative stress, and autophagy attenuated by Astragalus polysaccharides in mouse testis.

Xiao Y, Liu R, Tang W, Yang C
Reproductive toxicology (Elmsford, N.Y.) 2024 Jan;123:108520

The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats.

Zhang M, Zhang Q, Zhao W, Chen X, Zhang Y
Toxicology letters 2024 Feb;392:1-11

Sacral Nerve Stimulation Alleviates Intestinal Inflammation Through Regulating the Autophagy of Macrophages and Activating the Inflammasome Mediated by a Cholinergic Antiinflammatory Pathway in Colitis Rats.

Zhang S, Zhang C, Yan H, Yang L, Shi N, Liu C, Chen Y
Neuromodulation : journal of the International Neuromodulation Society 2024 Feb;27(2):302-311

Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland.

Zhang Y, Liu J, Niu G, Wu Q, Cao B
Developmental biology 2023 Sep;501:104-110

Lower dose of metformin combined with artesunate induced autophagy-dependent apoptosis of glioblastoma by activating ROS-AMPK-mTOR axis.

Ding W, Liao L, Liu J, Zhao J, Tang Q, Liao Y
Experimental cell research 2023 Sep 1;430(1):113691

MiR-155 enhances phagocytosis of alveolar macrophages through the mTORC2/RhoA pathway.

Yang X, Zeng X, Shu J, Bao H, Liu X
Medicine 2023 Sep 1;102(35):e34592

A Triple-Targeted Rutin-Based Self-Assembled Delivery Vector for Treating Ischemic Stroke by Vascular Normalization and Anti-Inflammation via ACE2/Ang1-7 Signaling.

Zhao T, He F, Zhao K, Yuxia L, Li H, Liu X, Cen J, Duan S
ACS central science 2023 Jun 28;9(6):1180-1199

Effects of lactate on metabolism and differentiation of CD4(+)T cells.

Zhang YT, Xing ML, Fang HH, Li WD, Wu L, Chen ZP
Molecular immunology 2023 Feb;154:96-107

The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma.

Sun Y, Yang X, Guan S, Ma T, Jiang Z, Gao M, Xu Y, Cong B
Archives of oral biology 2023 Dec;156:105810

A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation.

Cen J, Zhang R, Zhao T, Zhang X, Zhang C, Cui J, Zhao K, Duan S, Guo Y
Advanced healthcare materials 2022 Nov;11(22):e2200817

Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo.

Ma L, Zhang M, Cao F, Han J, Han P, Wu Y, Deng R, Zhang G, An X, Zhang L, Song Y, Cao B
Journal of cellular and molecular medicine 2022 May;26(9):2543-2556

miR-1 Regulates Differentiation and Proliferation of Goat Hair Follicle Stem Cells by Targeting IGF1R and LEF1 Genes.

Yan H, Jin M, Li Y, Gao Y, Ding Q, Wang X, Zeng W, Chen Y
DNA and cell biology 2022 Feb;41(2):190-201

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling.

Zhu H, Tong S, Yan C, Zhou A, Wang M, Li C
Immunopharmacology and immunotoxicology 2022 Dec;44(6):894-901

The clinicopathological and prognostic significance of mTOR and p-mTOR expression in patients with non-small cell lung cancer: A meta-analysis.

Qiu W, Ren M, Wang C, Fu Y, Liu Y
Medicine 2022 Dec 23;101(51):e32340

The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.

Zhang M, Ding L, Zhou Z, Liu C, Wang C, Chen B, Chen X, Zhang Y
Toxicology 2022 Aug;478:153290

Polyphyllin I Promotes Autophagic Cell Death and Apoptosis of Colon Cancer Cells via the ROS-Inhibited AKT/mTOR Pathway.

Luo Q, Jia L, Huang C, Qi Q, Jahangir A, Xia Y, Liu W, Shi R, Tang L, Chen Z
International journal of molecular sciences 2022 Aug 19;23(16)

Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway.

Tian G, Hu K, Qiu S, Xie Y, Cao Y, Ni S, Zhang L
Experimental and therapeutic medicine 2021 Nov;22(5):1304

Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells.

Zhang Y, Wu Q, Liu J, An X, Cao B
Genes 2021 Apr 29;12(5)

A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.

Zhang Y, Liu J, Li W, Cao F, Niu G, Ji S, Du X, Cao B, An X
Frontiers in cell and developmental biology 2020;8:383

Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.

Wu Y, Ma J, Sun Y, Tang M, Kong L
Chemosphere 2020 Sep;255:126913

MiR-29 regulates the function of goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways.

Wang P, Liu S, Zhu C, Duan Q, Jiang Y, Gao K, Bu Q, Cao B, An X
The Journal of steroid biochemistry and molecular biology 2020 Sep;202:105722

MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1.

Hu S, Liu YM, Chen-Chen, Li LY, Zhang BY, Yang JF, Li HD, Meng XM, Jun-Li, Xu T, Zhou H
Life sciences 2020 Oct 1;258:118147

Circular RNA hsa_circ_0010882 promotes the progression of gastric cancer via regulation of the PI3K/Akt/mTOR signaling pathway.

Peng YK, Pu K, Su HX, Zhang J, Zheng Y, Ji R, Guo QH, Wang YP, Guan QL, Zhou YN
European review for medical and pharmacological sciences 2020 Feb;24(3):1142-1151

miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.

Liu X, Zhang L, Yang L, Cui J, Che S, Liu Y, Han J, An X, Cao B, Song Y
Journal of cellular physiology 2020 Dec;235(12):10051-10067

Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling.

Song Z, Wei D, Chen Y, Chen L, Bian Y, Shen Y, Chen J, Pan Y
Toxicology and applied pharmacology 2019 Feb 1;364:45-54

Lactone Component From Ligusticum chuanxiong Alleviates Myocardial Ischemia Injury Through Inhibiting Autophagy.

Wang G, Dai G, Song J, Zhu M, Liu Y, Hou X, Ke Z, Zhou Y, Qiu H, Wang F, Jiang N, Jia X, Feng L
Frontiers in pharmacology 2018;9:301

Circ-8073 regulates CEP55 by sponging miR-449a to promote caprine endometrial epithelial cells proliferation via the PI3K/AKT/mTOR pathway.

Liu X, Zhang L, Liu Y, Cui J, Che S, An X, Song Y, Cao B
Biochimica et biophysica acta. Molecular cell research 2018 Aug;1865(8):1130-1147

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Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of mTOR expression in Jurkat cell lysate (M00003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody (Catalog # M00003) overnight at 4
Additional image