M00003

antibody from Boster Biological Technology

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00003

Provider

Boster Biological Technology

Product name

Anti-mTOR/Tor Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for TOR/MTOR detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry. Reactive species: Human, Mouse, Rat TOR/MTOR information: Molecular Weight: 288892 MW; Subcellular Localization: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137); Tissue Specificity: Expressed in numerous tissues, with highest levels in testis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CBD-13

Vial size

100ug/vial

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of mTOR expression in Jurkat cell lysate (M00003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody (Catalog # M00003) overnight at 4

Additional image