P00003
antibody from Boster Biological Technology
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- P00003 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for TOR/MTOR detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, PIg TOR/MTOR information: Molecular Weight: 288892 MW; Subcellular Localization: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB (PubMed:18497260, PubMed:20381137); Tissue Specificity: Expressed in numerous tissues, with highest levels in testis.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Antibody clone number
- IFF-13
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats.
Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland.
Effects of lactate on metabolism and differentiation of CD4(+)T cells.
The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma.
Moxidectin induces autophagy arrest in colorectal cancer.
Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo.
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling.
The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.
Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway.
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.
Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells.
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.
Moxidectin induces Cytostatic Autophagic Cell Death of Glioma Cells through inhibiting the AKT/mTOR Signalling Pathway.
Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
MiR-29 regulates the function of goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways.
MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1.
Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells.
CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells.
miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling.
Ivermectin induces autophagy-mediated cell death through the AKT/mTOR signaling pathway in glioma cells.
Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs).
Rapamycin provides anti-epileptogenic effect in a rat model of post-traumatic epilepsy via deactivation of mTOR signaling pathway.
Circ-8073 regulates CEP55 by sponging miR-449a to promote caprine endometrial epithelial cells proliferation via the PI3K/AKT/mTOR pathway.
Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway.
Zhang M, Zhang Q, Zhao W, Chen X, Zhang Y
Toxicology letters 2024 Feb;392:1-11
Toxicology letters 2024 Feb;392:1-11
Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland.
Zhang Y, Liu J, Niu G, Wu Q, Cao B
Developmental biology 2023 Sep;501:104-110
Developmental biology 2023 Sep;501:104-110
Effects of lactate on metabolism and differentiation of CD4(+)T cells.
Zhang YT, Xing ML, Fang HH, Li WD, Wu L, Chen ZP
Molecular immunology 2023 Feb;154:96-107
Molecular immunology 2023 Feb;154:96-107
The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma.
Sun Y, Yang X, Guan S, Ma T, Jiang Z, Gao M, Xu Y, Cong B
Archives of oral biology 2023 Dec;156:105810
Archives of oral biology 2023 Dec;156:105810
Moxidectin induces autophagy arrest in colorectal cancer.
Mao Y, Xie H, Shu D, Cheng L, Lan J, Pan K
Medical oncology (Northwood, London, England) 2022 Sep 29;39(12):211
Medical oncology (Northwood, London, England) 2022 Sep 29;39(12):211
Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo.
Ma L, Zhang M, Cao F, Han J, Han P, Wu Y, Deng R, Zhang G, An X, Zhang L, Song Y, Cao B
Journal of cellular and molecular medicine 2022 May;26(9):2543-2556
Journal of cellular and molecular medicine 2022 May;26(9):2543-2556
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling.
Zhu H, Tong S, Yan C, Zhou A, Wang M, Li C
Immunopharmacology and immunotoxicology 2022 Dec;44(6):894-901
Immunopharmacology and immunotoxicology 2022 Dec;44(6):894-901
The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.
Zhang M, Ding L, Zhou Z, Liu C, Wang C, Chen B, Chen X, Zhang Y
Toxicology 2022 Aug;478:153290
Toxicology 2022 Aug;478:153290
Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway.
Tian G, Hu K, Qiu S, Xie Y, Cao Y, Ni S, Zhang L
Experimental and therapeutic medicine 2021 Nov;22(5):1304
Experimental and therapeutic medicine 2021 Nov;22(5):1304
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.
Zhang X, Zhang L, Chen Z, Li S, Che B, Wang N, Chen J, Xu C, Wei C
International journal of molecular medicine 2021 Mar;47(3)
International journal of molecular medicine 2021 Mar;47(3)
Circ-140/chi-miR-8516/STC1-MMP1 Regulates αs1-/β-Casein Secretion and Lipid Formation in Goat Mammary Epithelial Cells.
Zhang Y, Wu Q, Liu J, An X, Cao B
Genes 2021 Apr 29;12(5)
Genes 2021 Apr 29;12(5)
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
Kong L, Wu Y, Hu W, Liu L, Xue Y, Liang G
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.
Zhang Y, Liu J, Li W, Cao F, Niu G, Ji S, Du X, Cao B, An X
Frontiers in cell and developmental biology 2020;8:383
Frontiers in cell and developmental biology 2020;8:383
Moxidectin induces Cytostatic Autophagic Cell Death of Glioma Cells through inhibiting the AKT/mTOR Signalling Pathway.
Liu J, Liang H, Khilji S, Li H, Song D, Chen C, Wang X, Zhang Y, Zhao N, Li X, Gao A
Journal of Cancer 2020;11(19):5802-5811
Journal of Cancer 2020;11(19):5802-5811
Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
Wu Y, Ma J, Sun Y, Tang M, Kong L
Chemosphere 2020 Sep;255:126913
Chemosphere 2020 Sep;255:126913
MiR-29 regulates the function of goat granulosa cell by targeting PTX3 via the PI3K/AKT/mTOR and Erk1/2 signaling pathways.
Wang P, Liu S, Zhu C, Duan Q, Jiang Y, Gao K, Bu Q, Cao B, An X
The Journal of steroid biochemistry and molecular biology 2020 Sep;202:105722
The Journal of steroid biochemistry and molecular biology 2020 Sep;202:105722
MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1.
Hu S, Liu YM, Chen-Chen, Li LY, Zhang BY, Yang JF, Li HD, Meng XM, Jun-Li, Xu T, Zhou H
Life sciences 2020 Oct 1;258:118147
Life sciences 2020 Oct 1;258:118147
Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells.
Shao CS, Zhou XH, Zheng XX, Huang Q
Journal of ethnopharmacology 2020 Nov 15;262:113213
Journal of ethnopharmacology 2020 Nov 15;262:113213
CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells.
Zhang M, Ma L, Liu Y, He Y, Li G, An X, Cao B
Genes 2020 Jun 28;11(7)
Genes 2020 Jun 28;11(7)
miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
Liu X, Zhang L, Yang L, Cui J, Che S, Liu Y, Han J, An X, Cao B, Song Y
Journal of cellular physiology 2020 Dec;235(12):10051-10067
Journal of cellular physiology 2020 Dec;235(12):10051-10067
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Wang P, Zhang SD, Jiao J, Wang W, Yu L, Zhao XL, Wang LT, Meng D, Fu YJ
Toxicology and applied pharmacology 2019 Sep 15;379:114647
Toxicology and applied pharmacology 2019 Sep 15;379:114647
Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling.
Song Z, Wei D, Chen Y, Chen L, Bian Y, Shen Y, Chen J, Pan Y
Toxicology and applied pharmacology 2019 Feb 1;364:45-54
Toxicology and applied pharmacology 2019 Feb 1;364:45-54
Ivermectin induces autophagy-mediated cell death through the AKT/mTOR signaling pathway in glioma cells.
Liu J, Liang H, Chen C, Wang X, Qu F, Wang H, Yang K, Wang Q, Zhao N, Meng J, Gao A
Bioscience reports 2019 Dec 20;39(12)
Bioscience reports 2019 Dec 20;39(12)
Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs).
Chen K, Hou J, Song Y, Zhang X, Liu Y, Zhang G, Wen K, Ma H, Li G, Cao B, An X
BMC veterinary research 2018 Nov 27;14(1):369
BMC veterinary research 2018 Nov 27;14(1):369
Rapamycin provides anti-epileptogenic effect in a rat model of post-traumatic epilepsy via deactivation of mTOR signaling pathway.
Wang F, Chen F, Wang G, Wei S, Fang F, Kang D, Lin Y
Experimental and therapeutic medicine 2018 Jun;15(6):4763-4770
Experimental and therapeutic medicine 2018 Jun;15(6):4763-4770
Circ-8073 regulates CEP55 by sponging miR-449a to promote caprine endometrial epithelial cells proliferation via the PI3K/AKT/mTOR pathway.
Liu X, Zhang L, Liu Y, Cui J, Che S, An X, Song Y, Cao B
Biochimica et biophysica acta. Molecular cell research 2018 Aug;1865(8):1130-1147
Biochimica et biophysica acta. Molecular cell research 2018 Aug;1865(8):1130-1147
Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway.
Shan M, Qin J, Jin F, Han X, Guan H, Li X, Zhang J, Zhang H, Wang Y
Free radical biology & medicine 2017 Sep;110:432-443
Free radical biology & medicine 2017 Sep;110:432-443
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate (P00003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody (Catalog # P00003) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR
- Additional image