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LearnNB600-607
antibody from Novus Biologicals
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB600-607 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-607, RRID:AB_10003186
- Product name
- Rabbit Polyclonal TOR/mTOR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reactivity occurs with phosphorylated mTOR from human derived tissues and cells.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Phospho-mTOR expression in human glioblastoma microglia-macrophage cells.
Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line.
SYK Targeting Represents a Potential Therapeutic Option for Relapsed Resistant Pediatric ETV6-RUNX1 B-Acute Lymphoblastic Leukemia Patients.
Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer.
Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/B polarized cells.
The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype.
The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes.
A proliferation-inducing ligand mediates follicular lymphoma B-cell proliferation and cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation.
Lisi L, Ciotti GMP, Chiavari M, Pizzoferrato M, Mangiola A, Kalinin S, Feinstein DL, Navarra P
Neurochemistry international 2019 Oct;129:104485
Neurochemistry international 2019 Oct;129:104485
Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line.
Chiavari M, Ciotti GMP, Navarra P, Lisi L
Brain sciences 2019 May 15;9(5)
Brain sciences 2019 May 15;9(5)
SYK Targeting Represents a Potential Therapeutic Option for Relapsed Resistant Pediatric ETV6-RUNX1 B-Acute Lymphoblastic Leukemia Patients.
Serafin V, Porcù E, Cortese G, Mariotto E, Veltri G, Bresolin S, Basso G, Accordi B
International journal of molecular sciences 2019 Dec 7;20(24)
International journal of molecular sciences 2019 Dec 7;20(24)
Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer.
Zhou X, Yue GG, Chan AM, Tsui SK, Fung KP, Sun H, Pu J, Lau CB
Biochemical pharmacology 2017 Oct 15;142:58-70
Biochemical pharmacology 2017 Oct 15;142:58-70
Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/B polarized cells.
Lisi L, Stigliano E, Lauriola L, Navarra P, Dello Russo C
ASN neuro 2014 May 8;6(3):171-83
ASN neuro 2014 May 8;6(3):171-83
The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype.
Lisi L, Laudati E, Navarra P, Dello Russo C
Journal of neuroinflammation 2014 Jul 23;11:125
Journal of neuroinflammation 2014 Jul 23;11:125
The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes.
Lisi L, Navarra P, Feinstein DL, Dello Russo C
Journal of neuroinflammation 2011 Jan 5;8(1):1
Journal of neuroinflammation 2011 Jan 5;8(1):1
A proliferation-inducing ligand mediates follicular lymphoma B-cell proliferation and cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation.
Gupta M, Dillon SR, Ziesmer SC, Feldman AL, Witzig TE, Ansell SM, Cerhan JR, Novak AJ
Blood 2009 May 21;113(21):5206-16
Blood 2009 May 21;113(21):5206-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
