ABIN501014

antibody from antibodies-online

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN501014

Provider

antibodies-online

Product name

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody

Antibody type

Polyclonal

Antigen

TOR antibody was raised against a 15 amino acid peptide from near the amino terminus ofhuman TOR.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing. Shelf life: one year from despatch.

References

Submitted references

Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression.

Fingar DC, Blenis J
Oncogene 2004 Apr 19;23(18):3151-71

---

Integration of growth factor and nutrient signaling: implications for cancer biology.

Shamji AF, Nghiem P, Schreiber SL
Molecular cell 2003 Aug;12(2):271-80

---

Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin.

Cardenas ME, Zhu D, Heitman J
Current opinion in nephrology and hypertension 1995 Nov;4(6):472-7

---

RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs.

Sabatini DM, Erdjument-Bromage H, Lui M, Tempst P, Snyder SH
Cell 1994 Jul 15;78(1):35-43

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of TOR in L1210 cell lysate with AP30931PU-N at (A) 1 and (B) 2 ?g/ml.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunocytochemistry of TOR in L1210 cells with AP30931PU-N at 2 ?g/ml.