PA5-13263
antibody from Invitrogen Antibodies
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-13263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mTOR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas.
Picot S, Faury N, Arzul I, Chollet B, Renault T, Morga B
Autophagy 2020 Nov;16(11):2017-2035
Autophagy 2020 Nov;16(11):2017-2035
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FRAP1 (arrow) using a mTOR polyclonal antibody (Product # PA5-13263) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a mTOR polyclonal antibody (Product # PA5-13263) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue using a mTOR polyclonal antibody (Product # PA5-13263), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7. Immunohistochemical detection of BECN1, MTOR, SQSTM1 and MAP1LC3A in several tissues of the Pacific oyster, Crassostrea gigas . (A) Mantle with connective tissue, nerf and muscle, (B) gonad, (C) gills, (D) digestive tubules, (E) muscle, (F) digestive gland epithelium, (G) mantle with connective tissue, (H) nerf in the mantle connective tissue, (I) gills, (J) digestive tubules, (K) mantle with connective tissue, (L) muscle, (M) digestive gland epithelium and connective tissue, (N) digestive tubule, (O) mantle with connective tissue, nerf and muscle, (P) gonad, (Q) gills, (R) digestive tubules, (S) mantle with nerf and muscle, (T) gonad, (U) gills and (V) digestive tubule. Scale bar: 20 um