Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX22732 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22732, RRID:AB_370514
- Product name
- mTOR antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
- Storage
- Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed using GTX22732 (1:1000 dilution) for use in Kinase Assays. The sample was recombinant 4E-BP1, incubated at 30¢J with mTOR immunoprecipitate and 32P-gamma ATP. Detection was performed using fluorography.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- "Western Blot: mTOR Antibody (GTX22732) - Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 μg) cells. Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 μg/ml (A) used for IP at 3 μg/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies. For blotting immunoprecipitated mTOR, GTX22732 was used at 1 μg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B)."
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTX22732 at a 1:100 dilutionconfocally stainingmTOR (red)in L6 myotubes,alongside a nuclear antigen antibody(green).