Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB1665 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-TOR (S2448) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Phospho-TOR (S2448) in ELISAs.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P42345
- Isotype
- IgG
- Antibody clone number
- 834115
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey.
Theaflavin-3, 3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and Notch-1 pathways, not via MAPK pathways.
Lane MT, Herda TJ, Fry AC, Cooper MA, Andre MJ, Gallagher PM
Biology of sport 2017 Jun;34(2):197-203
Biology of sport 2017 Jun;34(2):197-203
Theaflavin-3, 3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and Notch-1 pathways, not via MAPK pathways.
Gao Y, Rankin GO, Tu Y, Chen YC
International journal of oncology 2016 Jan;48(1):281-92
International journal of oncology 2016 Jan;48(1):281-92
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-TOR (S2448) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.