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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX60366 - Provider product page
- Provider
- GeneTex
- Product name
- HDAC3 antibody [GT9007]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of HeLa cells using HDAC3 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10¡¦ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500 (red). The nuclei were stained with DAPI.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ChIP was performed with HeLa nuclear extract and either 5 £gg of HDAC3 antibody or control mouse IgG. The precipitated DNA was detected by QPCR with primer set targeting to p21 or GAPDH promoter.
