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Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- 49-1042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HDAC3 Monoclonal Antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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- Invitrogen Antibodies (provider)
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- Experimental details
- ChIP assays were performed using human HeLa cells, the anti-HDAC3 antibody (Product # 49-1042) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 10,000 cells. Respectively, 5 μL of the HDAC3 monoclonal antibody and 5 μg of IgG (negative IP control) were used. QPCR was performed with primers for the promoters of the active genes c-fos antibody (Product # 49-1042) and GAPDH, and for the coding region of p21, a known target gene of HDAC3. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
