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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 685205 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#685205, RRID:AB_2687106
- Product name
- Direct-Blot™ HRP anti-HDAC3
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antibody clone number
- W15097A
- Vial size
- 100 µl (20 Western blots)
- Storage
- -20°C
- Handling
- Blue Ice
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate (15µg protein) from HeLa cells was resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1:1000 purified anti-HDAC3 or 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti-HDAC3 Antibody (upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
