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Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-59185 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HDAC3 Antibody
- Antibody type
- Monoclonal
- Description
- Concentrated Supernatant.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chromatin Immunoprecipitation: HDAC3 Antibody (C.15100145) [NBP2-59185] - ChIP assays were performed using human HeLa cells, the antibody against HDAC3 and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell number ChIP kit, on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. Respectively, 5 uL of the HDAC3 monoclonal antibody and 5 ug of IgG (negative IP control) were used. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure shows the recovery, expressed as % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
