Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- STJ98130 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-HDAC3 antibody (224-428) [3A7B5] (STJ98130)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Histone Deacetylase 3 (224-428) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis using HDAC3 monoclonal antibody against truncated Trx-HDAC3 recombinant protein (1) , full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate (2) and HeLa cell lysate (3).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded human esophagus cancer (left) and breast carcinoma tissue (right) , showing nuclear localization with DAB staining using HDAC3 monoclonal antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA