Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-79798 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79798, RRID:AB_1108804
- Product name
- Rabbit Polyclonal HDAC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Pitx2-dependent occupancy by histone deacetylases is associated with T-box gene regulation in mammalian abdominal tissue.
Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment.
Inhibition of histone deacetylase class I but not class II is critical for the sensitization of leukemic cells to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis.
Hilton T, Gross MK, Kioussi C
The Journal of biological chemistry 2010 Apr 9;285(15):11129-42
The Journal of biological chemistry 2010 Apr 9;285(15):11129-42
Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment.
Ishii S, Kurasawa Y, Wong J, Yu-Lee LY
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 18;105(11):4179-84
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 18;105(11):4179-84
Inhibition of histone deacetylase class I but not class II is critical for the sensitization of leukemic cells to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis.
Inoue S, Mai A, Dyer MJ, Cohen GM
Cancer research 2006 Jul 1;66(13):6785-92
Cancer research 2006 Jul 1;66(13):6785-92
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC3 Antibody [NB100-79798] - A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody NB100-79798 used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB