PA5-96776

antibody from Invitrogen Antibodies

Targeting: MAP2K4 JNKK1, MEK4, MKK4, PRKMK4, SERK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96776

Provider

Invitrogen Antibodies

Product name

MAP2K4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: BT-474, MCF7, SH-SY5Y, Rat skeletal muscle ; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.84 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MAP2K4 Polyclonal Antibody (Product # PA5-96776) and a 44 kDa band corresponding to MAP2K4 protein was observed across across all the cell lines. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), NIH/3T3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SEK1 in extracts of various cell lines using SEK1 Polyclonal Antibody (Product # PA5-96776) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SEK1 was performed in U2OS cells using SEK1 Polyclonal Antibody (Product # PA5-96776) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEK1 in paraffin-embedded human kidney using SEK1 Polyclonal Antibody (Product # PA5-96776) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of SEK1 was performed in 200 µg extracts of MCF-7 cells using SEK1 Polyclonal Antibody (Product # PA5-96776). Western blot was performed from the immunoprecipitate using SEK1 Polyclonal Antibody at a dilution of 1:1000.