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antibody from Invitrogen Antibodies
Targeting: MAP2K4
JNKK1, MEK4, MKK4, PRKMK4, SERK1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- CF500403 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAP2K4 Monoclonal Antibody (OTI8A8), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI8A8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K. Positive lysates LY401058 (100 µg) and LC401058 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-MAP2K4 mouse monoclonal antibody (TA500403) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4(RC206051).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-MAP2K4 mouse monoclonal antibody (TA500403) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4(RC206051).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (TA500403).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206051 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (TA500403), and then analyzed by flow cytometry.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
